Immunoelectron Microscopic Demonstration of Cortical Granule Lectins in Coelomic, Unfertilized and Fertilized Eggs of Xenopus laevis. (Xenopus eggs/polyspermy block/cortical granule lectin/immunoelectron microscopy)
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چکیده
منابع مشابه
Cortical grafting in Xenopus laevis.
T H E amphibian egg bears a cortex as the periphery of its cytoplasm. The interpretation by Dalcq & Pasteels (1937) of experiments performed by Pasteels (1938, 1940, 1941), in which fertile eggs of Rana fusca were centrifuged or inverted, led to the suggestion that the cortex possesses properties which control the course of development to some degree. It appeared that the properties of the cort...
متن کاملActivators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs
Prophase I oocytes, free of follicle cells, and metaphase II eggs of the amphibian Xenopus laevis were subjected to transient treatments with the protein kinase C activators, phorbol 12-myristate 13-acetate (PMA), phorbol 12,13-didecanoate, and 1-olyeoyl-2-acetyl-sn-glycerol. In both oocytes and eggs, these treatments triggered early events of amphibian development: cortical granule exocytosis,...
متن کاملPersistence, methylation and expression of vitellogenin gene derivatives after injection into fertilized eggs of Xenopus laevis.
We report the fate of different derivatives of the vitellogenin genes after injection into fertilized eggs of Xenopus. We injected a constructed minigene as well as a 5' fragment of the A2 vitellogenin gene. The minigene survives in embryogenesis much better than the 5' A2 fragment and is retained more frequently and at a higher level in frog tissues. The mosaic distribution of the foreign DNA ...
متن کاملPurification of a DNA-binding protein from Xenopus laevis unfertilized eggs.
A DNA-binding protein from Xenopus laevis unfertilized eggs has been purified to apparent homogeneity. It is a heat stable, lysine-rich protein and has a molecular weight corresponding to 8,200 daltons, measured by sodium dodecyl sulphate gel electrophoresis. The protein, which is active in a monomeric form, stimulates DNA polymerase alpha, and binds to single and double stranded DNA. One egg c...
متن کاملAgglutination of jelly coat and cortical granule components and the block to polyspermy in the amphibian Xenopus laevis.
A block to polyspermy in amphibians is established at fertilization by the conversion of the vitelline envelope to the fertilization envelope. In Xenopus laevis a major ultrastructural change in the envelope at fertilization is the appearance of an electron-dense layer, termed the F layer, between the envelope and the inner-most jelly coat layer, J(1). The F layer is derived, at least in part, ...
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ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1989
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1989.00325.x